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Citation (#335):

Antifungal spectrum and fungicidal mechanism of an N-terminal peptide of bovine lactoferrin

Authors: Wakabayashi, H., Hiratani, T., Uchida, K., Yamaguchi, H.

Journal: Journal of Infection and Chemotherapy 1996, 1(3): 185-189.

Abstract

The antifungal spectrum and fungicidal mechanism of an N-terminal peptide of bovine lactoferrin (lactoferricin |
B), an antimicrobial peptide produced by gastric pepsin digestion of bovine lactoferrin, were investigated. The
susceptibility of pathogenic yeasts and dermatophytes to the peptide varied in a species-dependent and straindependent
manner. Dematiaceous fungi and dimorphic fungi were susceptible to the peptide (range of MIC
values: 0.63 to 10 #g/mL). In the case of nonpigmented hyphomycetes and zygomycetes, most strains exhibited
resistance to the peptide (MIC: > 80 #g/mL). This peptide killed Candida albicans dose dependently
without inducing a change in cell wall stability against osmotic stress. The peptide at 10 #g/ml immediately
induced the release of K from C albicans cells and pH increases in cell suspensions. These pharmacological
activities were more potent than those for miconazole nitrate, a well-known antifungal agent that interferes
with membrane synthesis and function. These in vitro findings suggest that the lactoferrin oligopeptide has
potent membrane disrupting activity against this yeast and suggests that in vivo LF-B studies would be useful to
further understand host defenses and to develop improved therapeutic agents against yeast infections.

Keywords: lactoferrin; lactoferricin; Candida albicans; membrane disruption

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